赛特生物及美国 Cytelligen 公司作为用于分离人体及肿瘤动物模型体内循环肿瘤细胞 (CTC) 的差相富集技术 (Subsection Enrichment, SE)  及用于检测 CTC 的原位免疫荧光染色-FISH ( i•FISH ) 整合技术的首创发明人,已获得了中华人民共和国国家知识产权局及国家商标局颁发的唯一有关差相富集 CTC 技术以及 i•FISH 检测技术的发明专利证书及商标证书,同时也已获得了美国等国家政府机构颁发的国际性保护证书。
赛特生物的多种适用于不同生物医学实验的试剂与技术产品已在中国国家食品药品监督管理局 (CFDA) 注册
我们与国内外众多合作单位联合发表的与转化医学相关的部分文章与摘要(包括CTC,肿瘤细胞与分子生物学,脑神经学,内分泌学,免疫学,血液学,病原微生物学,C - 反应蛋白,免疫脂质体应用等)
X. Liu, J. Li, B. L. Cadilha, A. Markota, C. Voigt, Z. Huang, P. P. Lin, D. D. Wang, J. Dai,  G. Kranz, A. Krandick, D. Libl, H. Zitzelsberger, I. Zagorski, H. Braselmann, M. Pan, S. Zhu,Y. Huang, S. Niedermeyer, C. A. Reichel, B. Uhl, D. Briukhovetska, J. Suárez, S. Kobold, O. Gires, and H. Wang (2019) Epithelial-type systemic breast carcinoma cells with a restricted mesenchymal transition are a major source of metastasis (SA Highlights). Science Advances 5, eaav4275
Y. Li, X. Zhang, D. Liu, J. Gong, D. Wang, S. Li, Z. Peng, Y. Li, X. Wang, P. Lin, M. Li, and L. Shen (2018) Evolutionary expression of HER2 conferred by chromosome aneuploidy on circulating gastric cancer cells contributes to developing targeted and chemotherapeutic resistance (CCR Highlights) Clin Cancer Res 24:5261-71
P. Lin, J. Li, D. Wang, O. Gires, H. Wang  (2018) EpCAM differentially expressed on circulating and disseminated tumor cells promotes tumor metastasis in breast cancer patients Cancer Res 78:1059a
J. Zhang, H. Shi, T. Jiang, Z. Liu, P.P. Lin, and N. Chen (2018) Circulating tumor cells with karyotyping as a novel biomarker for diagnosis and treatment of nasopharyngeal carcinoma BMC Cancer 18:1133
P. Lin (2018) Aneuploid CTC and CEC Diagnostics 8(2):26(1-13)
L. Wang, Y. Li, J. Xu, A. Zhang, X. Wang, R. Tang, X. Zhang, H. Yin, M. Liu, D. Wang, P. Lin, L. Shen, J. Dong (2018) Quantified postsurgical small cell size CTCs and EpCAM+ circulating tumor stem cells with cytogenetic abnormalities in hepatocellular carcinoma patients determine cancer relapse Cancer Lett 412:99-107
X. Li, Y. Zhang, J. Ding, M. Wang, N. Li, H. Yang, K. Wang, D. Wang, P. Lin, M. Li, Z. Zhao, and P. Liu (2017) Clinical significance of detecting CSF-derived tumor cells in breast cancer patients with leptomeningeal metastasis Oncotarget 9:2705-14
P. Lin, O. Gires, D. Wang, L. Li and H. Wang (2017) Comprehensive in situ co-detection of aneuploid circulating endothelial and tumor cells. Sci Rep 7:9789
由磊,赵烨,阚秀,李一林,王东民,张爱群,王红霞,刘颖,王丹丹,林平,郑直 (2017) 同步原位检测CTC多重瘤标蛋白表达与染色体异倍体的意义及应用 临床与实验病理学杂志 (J Clin Exp Pathol) 33:297-302
Y. Li, X. Zhang, D. Liu, J. Gong, D.D. Wang, P.P. Lin, and L. Shen (2017) Dynamic acquisition of HER2 expression on circulating tumor cells in gastric cancer patients correlates to developing therapeutic resistance Cancer Res 77:3795a
H. Wang, N. Stoecklein, P. Lin and O. Gires (2017) Circulating and disseminated tumor cells: diagnostic tools and therapeutic targets in motion (Review) Oncotarget  8:1884-1912
Y. Li, X. Zhang, J. Gong, Q. Zhang, J. Gao, Y. Cao, D. D. Wang, P.P. Lin, and L. Shen (2016) Aneuploidy of chromosome 8 in circulating tumor cells correlates with prognosis in patients with advanced gastric cancer. Chin J Cancer Res 28:579-588
P. Lin (2016) NGS analysis of the single CTC or DTC isolated and subtyped by the integrated subtraction enrichment and immunostaining-FISHCancer Res 76:489a
P. Lin (2015) Integrated EpCAM‑independent subtraction enrichment and iFISH strategies to detect and classify disseminated and circulating tumors cells. Clin Transl Med 4(38):1-7
F. Ge, H. Zhang, D. Wang, L. Li and P. Lin (2015) Enhanced detection and comprehensive in situ phenotypic identification of circulating and disseminated heteroploid epithelial and glioma tumor cells. Oncotarget 6 (29): 27049-27064
P. Lin, L. Li, and D. Wang (2015) A novel integrated iFISH technology to detect and characterize non-hematopoietic heteroploid CTC subtypes with or without expressing vimentin or other tumor biomarkers. Cancer Res 75:557
J. Jiang, D. Wang,  M. Yang, D. Chen, L. Pang, S. Guo, J. Cai, J.P. Wery, L. Li, H. Li,and P. Lin (2015) Comprehensive characterization of chemotherapeutic efficacy on metastases in the established gastric neuroendocrine cancer patient derived xenograft model. Oncotarget  6(17):15639-15651
Y.Li, X. Zhang, S. Ge, J. Gao, J. Gong, M. Lu, Q. Zhang, Y. Cao, D. Wang, P. Lin, L. Shen (2014) Clinical Significance of Phenotyping and Karyotyping of Circulating Tumor Cells in Patients with Advanced Gastric Cancer. Oncotarget  5(16):6594-6602
R. Ran, L. Li, M. Wang, S. Wang, Z. Zheng, and P. Lin (2013) Determination of EGFR mutation in single cells microdissected from enriched lung tumor cells in peripheral blood. Anal. Bioanal. Chem. 405(23):7377-7382
J. Jiang, D.D. Wang, M. Yang, D. Chen, S. Guo, J. Cai, L. Li, J-P. Wery, P. Lin, and H. Li (2013) Metastatic PDX modeling displays correlation between CTC and metastatic potential, both inhibited by chemotherapy. Mol. Cancer Ther. 12:157
Q. Chen, F. Ge, W. Cui, F. Wang, Z. Yang, Y. Guo, L. Li, R. Bremner, and P. Lin (2013) Lung Cancer Circulating Tumor Cells Isolated by the EpCAM-Independent Enrichment Strategy Correlate with Cytokeratin 19-Derived CYFRA21-1 and Pathological Staging. Clin. Chim. Acta 419:57-61
X. Zhou, L. Li, H. Hao, S. Wang, Z. Guo, M. Wang, L. Zhang, W. Zhong, R. Ran, X. Zhang, L. Xu, and P. Lin (2010) Circulating tumor cells as a prognostic and predictive indicator in advanced lung cancer patients. Oncol. Progress 8(5):484-490
C. Wu, H. Hao, L. Li, X. Zhou, Z. Guo, L. Zhang, X. Zhang, W. Zhong, H. Guo, R. Bremner, and P. Lin (2009) Preliminary investigation of the clinical significance of detecting circulating tumor cells enriched from lung cancer patients. J. Thorac. Oncol. 4:30-36
P. Lin, C. Lavoie, T. Fischer, H. Huang, and M. Farquhar (2009) Calnuc plays a role in dynamic distribution of Galphai but not Gbeta subunits and modulates ACTH secretion in AtT-20 neuroendocrine secretory cells. Mol. Neurodegeneration. 4:1-16
L. Li, X. Zhou, H. Hao, M. Wang, Z. Guo, S. Wang, X. Xing, G. Harvie, E. Vuong, W. Shi, M. Tang, J. Rao, T. Reid, R. Bremner, P. Lin, J. Xu (2008) Detection of circulating tumor cells in newly diagnosed or relapsed lung cancer patients and the significance of CTC enumeration in patient staging. Cancer Res. 68:3691
D. Wang, Y, Liu, Y. Liu, C. Wang, H. Bao, H. Hao, S. Wang, Y. Zhang, H. Yu, X. Xing, G. Harvie, E. Vuong, J. Rao, T. Reid, P. Lin, J. Xu (2008) Enrichment and detection of circulating tumor cells in breast cancer patients Cancer Res. 68:3692
H. Guo, H. Hao, Z. Li, Y. Zhu, J. Sun, F. Ge, S. Wang, X. Xing, G. Harvie, E. Vuong, J. Rao, R. Bremner, T. Reid, P. Lin, J. Xu (2008) Preliminary investigation of lung cancer CTC distribution in patient body circulation Cancer Res. 68:3694
Y, Chen, H. Bao, C. Zhou, X. Xing, G. Harvie, E. Vuong, J. Rao, T. Reid, H. Qiu, Y. Xiao, H. Wu, M. Mei, C. Lu, P. Lin and J. Xu (2008) Detection of circulating tumor cell in patients with colorectal cancer and its potential implication. Cancer Res. 68:3696
X. Zhou, L. Li, M. Wang, L. Zhang, P. Lin and J. Xu (2008) Circulating tumor cells (CTC) as a prognostic and predictive factor in lung cancer patients. J Clin Oncol 26(15):22201
C. Ren, P. He, J. Zhang, Z. Zheng, Y. Xu, L. Zhou, H. Qiao, C. Wang, P. Zhao, W. Shi, M. Tang, P. Lin, and J. Xu (2007) Detecting circulating tumor cells in peripheral blood of pancreatic cancer patients using a negative selection strategy.Cancer Res. 67:5445
W. Shi, T. Reid, M. Tang, G. Harvie, H. Tao, G. Tao, P. Lin, and J. Xu (2007) Detection of circulating cancer cells from patient blood samples and correlation with anti-cancer therapy. Cancer Res. 67:5444
H. Yu, P. Lin, Y. Xu, Y. Zhou, Q. Sun, and J. Xu (2007) Breast cancer and circulating tumor cell (Review). Chn. J. Med. Lab. Tech. 8(3):215-218
Y. Chen, P. Lin, S. Qiu, XX. Peng, K. Loi, MG Farquhar and JY. Zhang (2007) Autoantibodies to Ca2+ binding protein Calnuc is a potential marker in colon cancer detection. Int. J. Oncol. 30(5):1137-44
P. Lin, F. Li, Y. Zhang, H. Huang, G. Tong, M. Farquhar and H. Xu (2007) Calnuc binds to Alzheimer’s beta-amyloid precursor protein and affects its biogenesis. J. Neurochem. 100:1505-1514
Y. Chen, P. Lin, K. Looi, R. Megliorino, and J. Zhang (2005) Autoantibodies to calcium binding protein Calnuc might be a potential marker in colon cancer detection. Cancer Res. 65:561
P. Lin, C. Ren, X. Zhao, L. Li, Y. Qiao , G. Harve, H. Tao, E. Vuong, G. Tao, M, Huang, L. Wu, and J. Xu (2005) Role of detecting circulating tumor cell in clinical cancer diagnosis and therapy. Chn. J. Lab. Tech. 1 (1):1-6
Y-W. Zhang, W-J Luo, H. Wang, P. Lin, K.S. Vetrivel, F. Liao, F. Li, P.C. Wong, M.G. Farquhar, G. Thinakaran, and H. Xu (2005) Nicastrin is critical for stability and trafficking but not association of other presenilin/g-secretases components. J. Biol. Chem. 280:17020-17026
C. Lavoie, T. Meerloo, P. Lin , and M.G. Farquhar (2002) Calnuc, an EF-Hand Ca2+-binding protein, is stored and processed in the Golgi and secreted by the constitutive-like pathway in AtT-20 cells. Mol. Endocrinol. 16:2462-2474
T.S.Weiss, C.E. Chamberlain, T. Takeda, P. Lin, K.M. Hahn, and M.G. Farquhar (2001) Galphai3 binding to calnuc on Golgi membranes in living cells monitored by fluorescence resonance energy transfer (FRET) of green fluorescent protein fusion proteins. Proc. Natl. Acad. Sci. USA. 98:14961-14966
P. Lin, T. Fischer, T. Weiss, and M.G. Farquhar (2000) Calnuc, an EF-hand Ca2+ binding protein, specifically interacts with the C-terminal alpha5-helix region of Galphai3. Proc. Natl. Acad. Sci. USA. 97:674-679
P. Lin, C. Lavoie, T. Fischer and M.G. Farquhar (2000) Calnuc regulates ACTH secretion in AtT-20 cells. Mol. Biol. Cell 11:50
P. Lin, Y. Yao, R. Hofmeister, R.Y. Tsien and M.G. Farquhar (1999) Overexpression of CALNUC (nucleobindin) increases agonist and thapsigargin relesable Ca2+ storage in the Golgi. J. Cell Biol. 145:279-289
P. Lin, T. Fischer, T. Weiss, and M.G. Farquhar (1999) Calnuc, an EF-hand Ca2+ binding protein, specifically interacts with the C-terminal region of Galphai3. Mol. Biol. Cell 10:418
P. Lin, H. Le-Niculescu, R. Hofmeister, J.M. McCaffery, M. Jin, H. Hennemann, T. McQuistan, L. De Vries, and M.G. Farquhar (1998) The mammalian Ca2+ binding protein, nucleobindin (CALNUC), is a Golgi resident protein. J. Cell Biol. 141:1515-1527
P. Lin, Y. Yao, R. Hofmeister, R.Y. Tsien and M.G. Farquhar (1998) Overexpression of CALNUC increases thapsigargin relesable Ca2+ storage in the Golgi. Mol. Biol. Cell 9:99
H. Le-Niculescu, P. Lin, M. Jin, H. Hennemann, D. Heinegard, T. McQuistan, J. M. McCaffery, N. Watson, L. De Vries, and M.G. Farquhar (1997) Characterization of a Golgi-associated EF-hand/ Ca2+ binding protein that interacts with heterotrimeric Galphai protein subunits. Mol. Biol. Cell 8:338
Y-P. Li, G. Curley, M. Lopez, M Chavez, R. Glew, A. Aragon, H. Kumar, and O.G. Baca (1996) Protein-tyrosine phosphatase activity of Coxiella Burnetii that inhibits human neutrophils. Acta Virologica 40:263-272
P. Lin, C. Mold, and T.W. DuClos (1995) Characterization of the C-reactive protein (CRP) receptor on the human monocytic cell line THP-1 (704). Immunol. Today 16(7):305-62
O.G. Baca, Y-P. Li, and H. Kumar  (1994) Survival of the Q fever agent Coxiella Burnetii in the phagolysosome. Trends in Microbiol. 2(12):476-480
Y-P. Li, C. Mold, and T.W. DuClos (1994) Sublytic Complement Attack Exposes C-Reactive Protein Biding Sites on Cell Membranes. J. Immunol. 152:2995-3005
T. Nakagaki, P. Lin, and W. Kisiel (1992) Activation of human factor VII by the prothrombin activator from the venom of Oxyuranus Scutellatus (taipan snake). Thrombosis Res. 65:105-116
P. Lin, Q.S. Lin, and S.Q. Nie (1992) Primary study of application of liposome immunolysis assay on drug monitoring. Prog. Clin. Biochem. p515-516. Editors: K. Miyai, T. Kanno, and E. Ishikawa. Excerpta Medica Publishing.
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